Abstract：Nickel （Ⅱ） could replace copper （Ⅱ）in Cu （Ⅱ）-EDTA complex and then coupled with luminol-H2O2-Cu （Ⅱ）chemiluminescence system. On the basis of this, a new determination method of nickel （Ⅱ）was established by replacement coupling reaction flow injection chemiluminescence method. The effects of Cu （Ⅱ）-EDTA complex ratio, medium and pH of reaction, the selection of oxidant and its concentration, and luminol concentration on relative chemiluminescence intensity were investigated. Interference ions were effectively masked by the addition of tartaric acid. Under the optimal experimental conditions, linear range of this method was 5.0×10-8-5.0×10-6 g/mL for nickel （Ⅱ）, and detection limit was 2×10-8 g/mL (3σ). The proposed method has been applied to the determination of nickel （Ⅱ） in environmental water samples, and the results were consistent with those obtained by atomic absorption spectrometry with relative standard deviations (RSD) of 3.7 %-4.6 %.
