Abstract：The color reaction of 5-(5-iodine-2-pyridylazo)-2,4-diaminotoluene (5-I-PADAT) and iridium （Ⅲ） was investigated by spectrophotometry. The results showed that iridium （Ⅲ） and 5-I-PADAT could form stable complex (1∶2) in HAc-NaAc buffer solution (pH 5.5-6.8). After acidification with strong acid, the complex could be converted to another protonized form with high absorption characteristic, significantly enhancing the sensitivity. In 1.2 mol/L of H2SO4 solution, the complex exhibited two absorption peaks at 542 nm and 584 nm，respectively. The apparent molar absorptivity wasε584=5.9×104 L·mol-1·cm-1. Beer's law was obeyed for iridium （Ⅲ） in the range of 0.03-1.0 μg/mL. The proposed method has been applied to the determination of iridium in catalyst. The result was consistent with the reference value and the relative standard deviation (RSD) was 0.52%.
杨晓慧，霍燕燕，郭阿娟，韩权. 5-(5-碘-2-吡啶偶氮)-2,4-二氨基甲苯与铱（Ⅲ）的显色反应及其应用[J]. 冶金分析, 2011, 31(9): 59-62.
YANG Xiao-hui, HUO Yan-yan, GUO A-juan, HAN Quan. Color reaction of 5-(5-iodine-2-pyridylazo)-2,4-diaminotoluene with iridium（Ⅲ）and its application. , 2011, 31(9): 59-62.
Brian D. Wagner and Gregory J. McManus .Enhancement of the fluorescence and stability of o-phthalaldehyde-derived isoindoles of amino acids using Hydroxypropyl-β-cyclodextrin[J]. Analytical .Biochemistry，2003， (317) ：233–239
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Raquel E.Galian,A.Guillermo Bracamonte,Alicia V.Veglia. Hydroxypropyl-β-cyclodextrin effect on the fluorescence of auxin and skatole and on the simultaneous determination of binary mixtures of indole compounds in urine by first derivativespectrofluorimetry[J].Analytic
