Abstract：The available phosphorus in calcareous soil was commonly determined by molybdenum-antimony anti-spectrophotometric method. The operation was complicated and the detection limit was high. Inductively coupled plasma atomic emission spectrometry (ICP-AES) combined with sodium hydrogen carbonate extracting-matrix masking was developed to effectively determine the available phosphorus in multitudinous soil samples. 0.5 mol/L sodium hydrogen carbonate solution at pH 8.5 was used as the leaching solution. The sample with water-to-soil ratio of 20∶1 (the ratio of leaching solution volume (mL) to soil mass (g)) was treated under oscillation at (25±1) ℃ for 30 min. After filtration with quantitative filter paper, 20.00 mL of filtrate was selected and acidified with 2 mL of (1+1) nitric acid. After the alkali metal ions were masked with 250 mg of succinic acid, phosphorus was determined by ICP-AES with P 214.914 nm as the analytical line. The results showed that the mass concentration of phosphorus had good linear relationship with its corresponding emission intensity in certain range. The linear correlation coefficient was 0.999 7. The limit of detection of method was 0.004 6 mg/kg, and the limit of quantification was 0.015 mg/kg. The proposed method was applied for the determination of available phosphorus in soil certified reference materials (GBW07413a, GBW07414a, GBW07459, GBW07460 and GBW07461), and the results were basically consistent with the certified values. The relative standard deviations (RSDs, n=6) of the determination results were between 0.87% and 2.1%. The content of available phosphorus in soil samples were analyzed according to the experimental method. The relative standard deviations (n=6) of determination results was not more than 1.7%. The results were basically consistent with those obtained by standard method of Ministry of Agriculture (NY/T 1121.7-2014). The two methods was no significant difference.
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