Aiming at the problems in simultaneous determination of major and minor components in chromite, the simultaneous determination method of 14 major and minor components in chromite (including Cr₂O₃, Fe, MgO, SiO₂, Al₂O₃, CaO, P, S, K₂O, Ni, Co, Ti, Mn and V) by X-ray fluorescence spectrometry with fusion sample preparation was established. Li₂B₄O₇-LiBO₂ (m∶m=67∶33) was used as flux with dilution ratio of 1∶20. The oxidation-releasing mixture (500g/L NaNO₃ solution-70g/L LiBr solution) was added quantitatively. The sample was pre-oxidized at 700℃ for 5min and then fused at 1100℃ for 20min. The as-prepared sample piece was transparent. The synthetic calibration sample series were prepared using certified reference materials of chromite, vanadium-titanium-iron concentrate and spectrum pure nickel oxide with different ratios. The content range of calibration curve was expanded. The detection limit of method was 10-748μg/g. The matrix effect was corrected by theoretical α coefficient method and empirical coefficient method. The precision test was conducted with one chromite sample. The relative standard deviations (RSD, n=12) of determination results were all less than 5%. The proposed method was applied for the analysis of one certified reference material of chromite, and the results were consistent with the certified values. The proposed method could meet the detection requirements of components in chromite.