Abstract：Micro Fe(III) showed obvious quenching effect on the fluorescence intensity of rhodamine B in NH3-NH4Cl buffer solution at pH 10.0. This quenching reaction was investigated and the optimal conditions for its application in Fe(III) analysis were discussed. In 25 mL colorimetric tube, 3.00 mL of rhodamine B solution, certain volume of Fe (III) standard solution and 2.00 mL of NH3-NH4Cl buffer solution at pH 10.0 were added. The solution was diluted to the mark with water. After shaking up, it was determined in 1 cm quartz cuvette which was pervious to light all around. The maximum emission wavelength and maximum excitation wavelength of system was λem=580 nm and λex=550 nm, respectively. The fluorescence quenching value (ΔF) showed linear relationship to the mass concentration of Fe(III) in range of 0.004-0.028 μg/mL with correlation coefficient of 0.987 9. The detection limit of method was 0.003 8 μg/mL. The proposed method was applied for the determination of Fe (III) in various environmental water samples (mineral water, spring water and tap water). The results were consistent with those obtained by atomic absorption spectrometry (GB 11911-1989 of national standard analysis method for water quality). The relative standard deviations (RSD, n=6) were between 3.5% and 7.5%. The recoveries were between 99% and 106%.
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