1.College of Chemistry and Materials Science,Hengyang Normal University,Hengyang 421008,China; 2.Key Laboratory of Functional Organometallic Materials, University of Hunan Province,Hengyang 421008,China
Abstract：3,5-dibromosalicylaldehyde rhodamine B hydrazide(RBDBH) fluorescence probe was synthesized and characterized by elemental analysis, infrared spectroscopy(IR) and nuclear magnetic resonance(NMR). The condition and application for fluorescence enhancement effect of mercury on RBDBH probe was systematically discussed. Based on this, a new fluorophotometric analysis method of mercury was established. After 25 μL of 1.000×10-3 mol/L RBDBH solution, proper amount of Hg2+ standard solution, 2.5 mL of acetonitrile and 2.2 mL of Tris-HCl buffer solution at pH 7.0 were added into 5 mL colorimetric tube successively and diluted to the mark with water, the determination was conductedwith 1 cm cuvette at excitation wavelength and emission wavelength of 520 nm and 570 nm, respectively. The results showed that: the concentraiton of mercury in range of 0.36-4.4 μmol/L showed good linearity to the corresponding fluorescence intensity with correlation coefficient as 0.997 4. The detection limit of method was 0.30 μmol/L. The RBDBH fluorescence probe was applied to the determination of mercury in waste water, and the results were basically consistent with those obtained by atomic absorption spectrometry(AAS). The relative standard deviation(RSD, n=5) were between 1.9% and 2.3%.
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