Investigation and application of cerium(Ⅲ)-Congo red-bovine serum albumin system by resonance Rayleigh scattering and resonance non-linear scattering
LI Guo-qiang1,2, HE Jia-hong1,2,XU Qiang*1,2
1.College of Materials and Chemical Engineering, Chongqing University of Arts and Sciences, Chongqing 402160, China; 2.Chongqing Key Laboratory of Environmental Materials and Remediation Technology, Chongqing University of Arts and Sciences,Chongqing 402160,China
Abstract：Based on the formation of ternary ion-association complex cerium(Ⅲ)-Congo red-bovine serum ablumin(Ce(Ⅲ)-CGR-BSA),a new resonance Rayleigh scattering and resonance non-linear scattering method has been developed for the determination of trace amounts of Ce(Ⅲ). It was discovered that in NH3-NH4Cl buffer solution at pH 7.6, Ce(Ⅲ) could react with Congo red (CGR) and bovine serum albumin (BSA) to form larger size and more hydrophobic ternary ion-association, which caused the resonance Rayleigh scattering (RRS), second-order scattering (SOS) and frequency double scattering (FDS) intensities dramatically enhanced.The maximum scattering wavelength of RRS, SOS and FDS were located at 278,543, 390 nm, respectively. Through condition test, it was determined that 0.6 mL of NH3-NH4Cl buffer solution at pH 7.6 was used as the reaction medium, 1.8 mL of 0.025 mg/mL CGR solution and 1.0 mL of 0.1 mg/mL BSA solution was used. The reaction time was 5 min, and the presence of a large amount of common coexisting ions had no interference with the determination of Ce(Ⅲ). Further investigation showed that the enhancement of scattering intensities (ΔIRRS,ΔISOS and ΔIFDS) was linear to the Ce(Ⅲ) mass concentration in the range of 2.0-10.0 μg/mL, and the detection limits (3σ) were 0.29 ng/mL for RRS, 0.91 ng/mL for SOS and 3.54 ng/mL for FDS. Moreover, the reaction mechanism and the reason for scattering enhancement were also discussed. The proposed method was applied to the determination of Ce(Ⅲ) in actual water sample, the relative standard deviation was 0.94%-1.2%. The found results were consistent with those obtained by atomic absorption spectrometry(AAS), the recovery was between 96% and 102%.
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