Determination of trace cobalt in fly ash by carbon dots fluorescence quenching method
LUO Dao-cheng1,2 , LUO Zhu1
1.School of Chemistry and Chemical Engineering,Hunan University of Science and Technology,Xiangtan 411201,China; 2.Hunan Provincal Key Laboratory of Clean Coal and Mine Environmental Protection,Xiangtan 411201,China
Abstract：Based on the significant quenching effect of cobalt ions on the water soluble fluorescent carbon dots (CDs) in B-R buffer solution at pH 6.80, a method was established for determination of cobalt ion by fluorescence quenching method. 0.5 mL of 3.6×10-4 mol/L fluorescent CDs solution(in carbon), 1.0 mL of B-R buffer solution at pH 6.80 and proper amount of cobalt standard working solution were added successively into 5 mL colorimetric tube and diluted to the mark. After reaction at room temperature for 10 min, the relative fluorescence intensity of test system was determined at 440 nm with 350 nm as excitation wavelength. The results showed that the concentration of cobalt ion in range of 2×10-6-7.6×10-5mol/L showed good linear relationship with the relative fluorescence intensity of CDs. The equation of linear regression was ΔF=1.008 7+0.031 25×10-6c (mol/L) with correlation coefficient of r=0.998 5. The detection limit of method was 1.2×10-7 mol/L. The proposed method was applied to the determination of trace cobalt in fly ash, and the results were consistent with those obtained by the standard method GB/T 15922-2010. The relative standard deviation (RSD, n=6) was 0.9%-1.0%. The recoveries were between 98% and 104%.
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