Establishment of absorbance variation of unit wavelength- spectrophotometry and its application in the detection of chromium in environmental samples
Abstract：The variation amplitude of absorbance of unit wavelength had certain relationship with the concentration of analyte within the range of absorption peak. Based on this, an absorbance variation of unit wavelength-spectrophotometry (ACW-S method) was established. Moreover, its function to reduce the influence of sample turbidity on detection results was discussed. The feasibility of ACW-S method was verified using chromium in environmental water as an example. The factors including detection limit, accuracy and precision were investigated. The wavelength range for fitting was screened. Total 13 wave bands were selected including 570-590 nm. The linear range of calibration curve of chromium was between 5.0 μg/L and 300 μg/L in these 13 wave bands. The detection limits of method were 0.7-1.0 μg/L. The recoveries for direct determination of actual samples were between 80.0% and 116 % (the mass concentration of added standard was 10.0 μg/L and 20.0 μg/L). The ACW-S method could reduce the positive interference caused by sample turbidity. It was suitable for the sample with absorbance less than 0.300 at 750 nm warelength after coloring. Moreover, the influence of sample turbidity on the determination results could be significantly reduced. The detection effect could be further improved by the combination of filtration method or turbidity compensation method.
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