1.College of Chemistry and Chemical Engineering, Zhoukou Normal University, Zhoukou 466001, China; 2. Research Institute of Pharmaceutical Chemistry, Zhoukou Normal University, Zhoukou 466001, China
Abstract：:A simple spectrofluorimetric method for the determination of selenium based on the fluorescence quenching of hemoglobin was developed.The influence of acidity, L-tyrosine concentration, H2O2 concentration,hemoglobin concentration and reaction time on the system were studied.In NH3·H2O-NH4Cl buffer solution of pH 9.8,when the concentration of L-tyrosine,H2O2 and hemoglobin were 7.5×10-5 mol/L,1.0×10-4 mol/L and 2.0×10-7 mol/L respectively,the linear range for the determination of selenium was 0.3-50 μg/mL with the detection limit of 0.05 μg/mL. The proposed method was applied for the determination of selenium in selenium-enriched soil, the results were consistent with those of atomic absorption spectrometry. The relative standard deviation (RSD) was 4.6%-4.9%.
Schermer S,Beinrohr E.Optimization of electrochemical hydride generation in a miniaturized electrolytic flow cell coupled to microwave-induced plasma atomic emission spectrometry for the determination of selenium [J].Fresenius J. Anal. Chem.,2001,371:740-745.
Zhang H,Chai Z F,Qing W Y,et al.Cyclic neutron activation analysis for determination of selenium in food samples using 77mSe [J].J. Radioanal. Nucl. Chem.,2009,281:23-26.
[15]
Kannamkumarath S S,Vonderheide A.HPLC-ICP-MS determination of selenium distribution and speciation in different types of nut [J].Anal. Bioanal. Chem.,2002,373:454-460.
[16]
Labat L.Rapid and simple determination of selenium in blood serum by inductively coupled plasma-mass spectrometry (ICP-MS) [J].Anal. Bioanal. Chem.,2003,376:270-273 .
[17]
Kobayashi K,Katsuya Y,Abdulah R,et al.Rapid and direct determination of selenium, copper and zinc in blood plasma by flow injection-inductively coupled plasma-mass spectrometry [J] .Humana. Press. Inc.,2007,115:87-93.
