Abstract：Fluorescence spectroscopy, synchronous fluorescence spectroscopy and UV-visible absorption spectroscopy were employed to study the spectral behavior of the binding reaction between MnS nanocrystals and gelatin at different temperatures at pH 12.0. The results showed that the MnS nanocrystals produced in the gelatin solution and gelatin combined to form complexes by van der Waals and hydrogen bond forces, which led to the endogenous fluorescence quenching of the gelatin (a static fluorescence quenching) while MnS nanocrystals didn’t cause any conformational changes to the gelatin tryptophan residues. According to the Lineweave-Burk equation for static quenching, the binding constant and thermodynamic parameters for MnS nanocrystals binded with gelatin at the temperatures of 293 K and 305 K were calculated, giving the binding constants as 4.72×103 L·mol-1 and 3.23 × 103 L·mol-1, respectively, the enthalpy change (ΔH) as －23.5 kJ·mol-1, the entropy change (ΔS) as -9.86 J·K-1·mol-1 and -9.87 J·K-1·mol-1, respectively and the Gibbs free energy changes (ΔG) as 20.61 kJ·mol-1 and 20.49 kJ·mol-1, respectively.
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WANG Huan, ZHANG Ping, WANG Xiao-ling. Investigation on interaction of nano-MnS and gelatin by fluorescence quenchingmethod combined with UV-visible absorption spectrometry. , 2014, 34(2): 1-5.
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