1.College of Chemistry and Chemical Engineering, Zhoukou Normal University, Zhoukou 466001, China ;2.Research Institute of Pharmaceutical Chemistry, Zhoukou Normal University, Zhoukou 466001, China
Abstract：Vanadium(V) had strong quenching effect on the catalytic spetrophotimetric system of hemoglobin analog enzyme. Based on this, a novel enzymatic spectrophotometry for determining vanadium(V) was established.The influence of acidity, rhodamine B concentration, H2O2 concentration, hemoglobin concentration and reaction time on the system was studied.In NH3·H2O-NH4Cl buffer solution of pH 9.8, when the concentration of rhodamine B, H2O2 and hemoglobin were 1.5×10-5 mol/L, 1.5×10-4 mol/L and 2.0×10-6mol/L respectively, the linear range for the determination of vanadium(V) was 3.1×10-4 - 6.2×10-3g/L with the detection limit of 2.1×10-6g/L.1 000 times of Na+, K+, Cl-, NO3－, NH4+, F－ and BrO3－, 500 times of Ca2+, Fe3+, Al3+, Mn2+, Ba2+ and CO32－, 100 times of C2O42－, 5 times of Cu2+ did not interfere the determination of vanadium(V). The RSD was 3.7% for 7 parallel determinations of vanadium(V) in environmental water.The results obtained by the extraction spectrophotometry and the proposed method are statistically compared. The t-test showed that the two methods had no significant differences.
Takeshi Y, Yuzuru Y.2-(5-nitro-2-pyridylazo)-5-(n-propyl-n-sulfopropylarnine) phenol as a new analytical reagent for flow-injection spectrometric determination of trace vanadium(V)[J] .Microchimica Acta, 1998, 130(1-2):111-115.
秦朝远, 钱冬梅, 何兴.不容忽视的生命微量元素[J] .甘肃科技(Gansu Science and Technology), 2005, 21(9):115-116.
[4]
Kiran Kumar T N, Revanasiddappa H D.Spectrophotometric determination of vanadium using variamine blue and its application to synthetic, environmental and biological samples[J] .Journal of the Iranian Chemical Society, 2005, 2(2):161-167.
王艳丽.催化动力学分析法测定超痕量钒的研究[J] .分析测试技术与仪器(Analysis and Testing Technology and Instruments), 2010, 16(1):42-47.
[8]
Jie N Q, Zhang Q, Wei Y J, et al.Study on the catalytic determination of vanadium(V) using the rhodamine 6G-Periodate redox reaction and its analytical application[J] .Microchimica Acta, 2002, 140(1-2):103-107.
[9]
尹继先, 朱霞萍, 梁庆勋, 等.微波消解-分光光度法测定钒钛磁铁矿中的钒[J] .岩矿测试(Rock and Mineral Analysis), 2010, 29(6):719-722.
[10]
Zhang Z Q, Liu X P, Zhan H Y.Flow injection catalytic determination of vanadium using the indicator reaction between victoria blue B and bromate and A citric acid activator [J] .Analytical Letters, 1999, 32(10):2115-2126.
Yaman M, Gucer S.Determination of vanadium in biological matrices by flame atomic absorption spectrometry with activated carbon enrichment[J] .Fresenius' Journal of Analytical Chemistry, 1994, 350(7-9) :504-509.
