Abstract：A new triazene reagent 1-(2-benzothiazole)-3-(3,5-dibromopyridyl)-triazene (BTPyBT) was synthesized and identified. The effect of conditions such as acidity, buffer solution dosage, copper ion concentration, reagent dosage, reaction temperature and reaction time on the fluorescence reaction of BTPyBT with copper was investigated. Based on this, a determination method of copper by fluorometry was established. The results showed that, BTPyBT reacted with copper at room temperature for 20 min to form a stable complex with composition ratio of 3∶1 in sodium borate-sodium hydroxide buffer solution at pH 9.16. The linear range of copper for the fluorometry was 0.5-80.0 μg/L. The determination limit was 0.2 μg/L. The proposed method has been applied to the determination of trace copper(Ⅱ) in mineral water, purified water and river water. The results were consist those obtained by atom absorption spectrometry, and the relative standard deviations (n=6) were 1.2%-4.7%.
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