Abstract： The determination of copper in a novel material (fire smelting nickel substrate material) by spectrophotometry was proposed. In ammonium chloride-ammonium hydroxide buffer solution at pH 8.9-9.4, the stable chelate formed by bisoxaldihydrazone (BCO) and Cu2+ had maximum absorption wavelength at 600 nm. The determination of copper was interfered by matrix iron, nickel, chromium and cobalt. However, after the sample was dissolved in hydrochloric acid, nitric acid and hydrofluoric acid and chromium volatilized with hydrochloric acid, the interference could be eliminated by matrix matching and citric acid masking method. The content of copper in four actual samples was determined by this method with relative standard deviation in the range of 1.4 % - 4.0 %. The atomic absorption spectrometry was used for comparison analysis. Meanwhile, the samples were also determined in three other laboratories, and the results were basically consistent.
［5］王学伦.铜—新亚铜灵—甲基橙三元络合物萃取分光光度法测定焊锡及金属锡中铜［J］.云锡科技（Yunnan Tin Technology），1989，16（3）：46-50.
［6］Ali Niazi,Ateasa,Yazdanipour. Simultaneous spectrophotometric determination of cobalt, copper and nickel using 1-（2-thiazolylazo）-2-naphthol by chemometrics methods［J］.Spectrochimica Acta:Chinese Chemical Letters，2008，19（7）：860-864.
［7］Li Yuan，Shu Hui,Huo Xiao Na, et al. A novel spectrophotometric determination of copperⅡ with bromosulphonazo Ⅲ［J］.Spectrochimica Acta:Chinese Chemical Letters，2008，19（1）：92-94.
［8］ 盛华栋. 电感耦合等离子体原子发射光谱法测定8种蔬菜中微量元素［J］.理化检验：化学分册（Physical Testing and Chemical Analysis: Part B Chemical Analysis）,2008,44(1):25-27.
［17］ Roldana Paulo S,Ialcantara lton L,Padilha Cilene CF.Determination of copper,iron,nickel and zinc in gasoline by FAAS after sorption and preconcentration on silica modified with 2-aminotiazole groups［J］.Fuel,2005,84(2):305-309.
