Abstract：Salt,potassium ions and ammonia nitrogen are the key factors affecting the anaerobic fermentation rate and high gas production rate.Therefore,the accurate determination of their contents is of great significance for guiding the fermentation process and waste resource utilization.The determination of salts,potassium and ammonia nitrogen in anaerobic fermentation broth was realized by ion chromatography under the follow experimental conditions:1.7 mmol/L nitric acid was used as the eluent,the pH was 2.60,the flow rate of eluent was 0.8 mL/min,and the column temperature was set at 30 ℃.The interference tests indicated that the coexisting ions in anaerobic fermentation broth did not influence on the determination.The correlation coefficients of standard curves were greater than 0.999 7,and the minimum detection concentrations were 0.000 80-0.002 5 mg/L.The contents of salts,potassium and ammonia nitrogen in fermentation broths of kitchen waste and straw were determined according to the experimental method. Meanwhile,the determination of potassium and sodium by inductively coupled plasma atomic emission spectrometry (ICP-AES),and the determination of ammonia nitrogen by salicylic acid spectrophotometry in GB/T 34500.5-2017 were also conducted for comparison.The determination results were analyzed by F test.The results showed that the measured values of experimental method were basically consistent with those obtained by the comparison methods.The relative standard deviations (RSD,n=6) of the determination results of proposed method were 0.38%-4.5%, while the RSDs of comparison methods were 0.71%-3.1%.The results of F＜F0.05,5=5.05 indicated that there was no significant difference in precision between the experimental method and comparison methods.The contents of salts,potassium and ammonia nitrogen in fermentation broths of four samples, i.e.,kitchen waste,straw,silage and Chinese Pennisetum,were determined according to the experimental method.Moreover,the recovery tests were conducted by adding the standard solution of each component to be measured.The recoveries were between 86% and 114%.
王树加, 颜舒畅, 王闻, 范炜林, 陈佩丽, 苏秋成. 离子色谱法测定厌氧发酵液中盐分、钾和氨氮[J]. 冶金分析, 2023, 43(7): 83-89.
WANG Shujia, YAN Shuchang, WANG Wen, FAN Weilin, CHEN Peili, SU Qiucheng. Determination of salts,potassium and ammonia nitrogen in anaerobic fermentation broth by ion chromatography. , 2023, 43(7): 83-89.
[1] PONSA S,GEA T,SANCHEZ A.Anaerobic co-digestion of the organic fraction of municipal solid waste with several pure organic co-substrates[J].Biosystems Engineering,2011(4):352-360. [2] 沃德芳,孙永明,余德才,等.温度和青贮对杂交狼尾草和餐厨垃圾混合厌氧发酵的影响[J].中国沼气,2021,39(4):39-46. WO Defang,SUN Yongming,YU Decai,et al.Effects of temperature and silaging on co-digestion of hybrid pennisetum and kitchen waste[J].China Biogas,2021,39(4):39-46. [3] 刘研萍,王玮,陈雪,等.盐分对餐厨垃圾厌氧消化的影响[J].中国沼气,2016,34(2):53-57. LIU Yanping,WANG Wei,CHEN Xue,et al.Effect of salinity concentration on anaerobic digestion of food waste[J].China Biogas,2016,34(2):53-57. [4] 许之扬,赵明星,缪恒峰,等.氨氮质量浓度对餐厨垃圾厌氧消化产沼气的影响[J].食品与生物技术学报,2014,33(3):282-287. XU Zhiyang,ZHAO Mingxing,LIAO Hengfeng,et al.Analysis of ammonia inhibition on anaerobic digestion via food wastes[J].Journal of Food Science and Biotechnology,2014,33(3):282-287. [5] 李江浩.玉米秸秆氢氧化钾及蒸汽爆破耦合预处理厌氧发酵产沼气研究[D].北京:北京化工大学,2015. [6] 王攀,李冰心,黄燕冰,等.含盐量对餐厨垃圾干式厌氧发酵的影响[J].环境污染与防治,2015,37(5):27-31. WANG Pan,LI Bingxin,HUANG Yanbing,et al.Effect of salt content on dry anaerobic fermentation for food waste[J].Environmental Pollution & Control,2015,37(5):27-31. [7] 王曼娟,嵇龙,蔡璐.X射线荧光光谱法测定含铁原料中钾、钠、锌含量[J].中国无机分析化学,2018,8(2):28-31. WANG Manjuan,JI Long,CAI Lu.Determination of potassium,sodium and zinc in ferrous matrerials by X-ray fluorescence spectrometry[J].Chinese Journal of Inorganic Analytical Chemistry,2018,8(2):28-31. [8] 李强,郑凯清,王彦美,等.离子色谱法测定海洋沉积物孔隙水中7种阳离子[J].冶金分析,2016,36(2):38-42. LI Qiang,ZHENG Kaiqing,WANG Yanmei,et al.De-termination of seven cations in pore water of ocean sediments by ion chromatography[J].Metallurgical Analysis,2016,36(2):38-42. [9] 王树加,陈晓丽,关进安,等.微波消解-电感耦合等离子体发射光谱(ICP-OES)法测定生物质中的碱金属和碱土金属[J].中国无机分析化学,2022,12(1):107-113. WANG Shujia,CHEN Xiaoli,GUAN Jin'an,et al.Determination of alkali metal and alkaline earth metal content in biomass by ICP-OES with microwave digestion[J].Chinese Journal of Inorganic Analytical Chemistry,2022,12(1):107-113. [10] 陈永欣,曾雪灵,黎香荣,等.免试剂离子色谱法测定入境冶炼渣浸出液中阴阳离子[J].冶金分析,2012,32(2):32-37. CHEN Yongxin,ZENG Xueling,LI Xiangrong,et al.Determination of anion and cation in leaching solution of imported smelting slag by reagent free ion chromatography[J].Metallurgical Analysis,2012,32(2):32-37. [11] 贾海峰,王峰,罗琦林,等.微波消解-离子色谱法测定磷矿石中氟和氯[J].冶金分析,2022,42(8):62-67. JIA Haifeng,WANG Feng,LUO Qilin,et al.Determiniation of fluorine and chlorine in phosphate ore by ion chromatography with microwave digestion[J].Metal-lurgical Analysis,2022,42(8):62-67. [12] 孙帅,夏海龙,林孔亮.离子色谱法测定地表水中NH+4-N[J].中国无机分析化学,2021,11(3):28-31. SUN Shuai,XIA Hailong,LIN Kongliang.Determination NH+4-N in surface water by ion chromatography[J].Chinese Journal of Inorganic Analytical Chemistry,2021,11(3):28-31. [13] 王树加,周肇秋,吴梁鹏,等.基于氧弹燃烧-离子色谱法测定生活垃圾可燃组分中氯和硫的含量[J].中国无机分析化学,2020,10(3):9-13. WANG Shujia,ZHOU Zhaoqiu,WU Liangpeng,et al.Determination of Cl and S in dry basis waste by mean of oxygen bomb combustion and ion chromatography[J].Chinese Journal of Inorganic Analytical Chemistry,2020,10(3):9-13. [14] 王树加,黎佩珊,陈晓丽,等.离子色谱仪检定及整机性能不确定度评定[J].化学分析计量,2021,30(1):67-71. WANG Shujia,LI Peishan,CHEN Xiaoli,et al.Verification of ion chromatograph and evaluation of uncertainty of overall performance[J].Chemical Analysis and Meterage,2021,30(1):67-71. [15] 万家亮,曾胜年,李俊义.分析化学[M].北京:高等教育出版社,2001.
