During the determination of copper in copper anode slime by iodometry according to the industrial standard method YS/T 745.1-2010, hydrobromic acid is used to remove interfering elements such as selenium, arsenic, antimony and tin. The sample dissolution process is tedious and time-consuming. The splashing occurs easily, leading to invalid results and low detection efficiency. So, it is not suitable for simultaneous and rapid analysis and detection of a large number of samples in smelting enterprises. In the experiment, hydrochloric acid was used to decompose the sample. After removing the impurities such as selenium by mixed nitric acid and sulfur acid, the impurity elements in sample was further removed using bromine instead of hydrobromic acid. Consequently, the determination method of copper in copper anode slime by iodometry according to YS/T 745.1-2010 was modified. The dosage of mixed nitric acid and sulfur acid was 15mL. Then the salts could be fully dissolved after adding 3mL of hydrochloric acid and dissolving the sample until the remaining volume of the solution was about 0.5mL. The content of copper in copper anode slime sample was determined according to the experimental method. The relative standard deviation (RSD, n=7) of the determination results was less than 0.50%. The found results of each sample were consistent with those obtained by industrial standard method YS/T 745.1-2010. There was no significant difference between two methods, and the proposed method could be applicable for the rapid analysis of copper in a large number of copper anode slime samples in smelting enterprises.