Abstract：Copper could react with rufloxacin hydrochloride (RH) in HAc-NaAc medium to form stable complex, which generates obvious fluorescence quenching phenomenon. Based on this, a new determination method of copper (Ⅱ) was established. The effect of solution acidity, surfactant, concentration and dosage of RH, and the adding order of reagents on system were investigated, and the optimized analytical conditions were obtained. The results showed that the fluorescence intensity had good linearity with the concentration of copper (Ⅱ) standard solution in the range of 2.25×10-8-2.5×10-6 mol/L at measuring wavelength of 520 nm. The detection limit was 9.6×10-9 mol/L. The proposed method was applied to the analysis of actual water sample, and the determination results were consistent with those found by atomic absorption spectrometry. The relative standard deviations (RSD) were 0.8 %-1.6 %.
