Abstract：A poly(L-cysteine)/acetylene black modified glassy carbon electrode (L-Cys/AB/GCE) was prepared through drop coating method and electrochemical polymerization method, and the interfacial property of its surface was investigated by electrochemical alternating current impedance and chronocoulometry. Furthermore, the electrochemical behavior of hydroquinone(HQ) was investigated by cyclic voltammetry(CV) on this modified electrode. The impedance graph indicated that the existence for the modified film of L-cys/AB/GCE could accelerate the transfer of electron. The chronocoulometry graph showed that the specific surface area of L-cysteine/AB/GCE was six times of that of bare electrode. The behavior of CV implied that the L-Cys/AB/GCE had an excellent electrocatalytic effect for the oxidation and reduction of HQ with four times in peak current response relative to the bare sensor, which improved the detection sensitivity of HQ. For L-Cys/AB/GCE, its peak current had a good linear relationship with the square root of scanning rate with correlation coefficient R2=0.995-0.998, indicating that the electrochemical reaction of HQ on L-Cys/AB/GCE was controlled by the process of diffuse velocity. Under optimized condition with 0.1 mol/L phosphate buffer solution (PBS) at pH 7.0 as the supporting electrolyte, the results of differential pulse voltammetry(DPV) indicated that the oxidation peak current(Ipa) of HQ showed a good linearity with its concentration(c) ranging from 0.5-1 000 μmol/L with a correlation coefficient(R) of 0.998 and detection limit of 0.08 μmol/L. Therefore, a new method for determination of HQ in water sample was established. When this method was used for HQ determination in environmental water sample, the results was consistent with those by high performance liquid chromatography, and the relative standard deviation (RSD, n=8) was 2.3%-2.6%.
许春萱,丁婷婷,杨媛媛,钟翔,卢先春. 聚L-半胱氨酸/乙炔黑修饰玻碳电极测定水样中对苯二酚[J]. 冶金分析, 2016, 36(9): 19-24.
XU Chun-xuan, DING Ting-ting, YANG Yuan-yuan, ZHONG Xiang, LU Xian-chun. Determination of hydroquinone in water with poly(L-cysteine)/acetylene black modified glassy carbon electrode. , 2016, 36(9): 19-24.
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