Abstract：The interaction between Cu2+ and 1-(3,4-dihydroxy benzoyl) pyrene-boric acid complex was investigated by fluorescence spectrometry and spectrophotometry. The results showed that Cu2+ had fluorescence quenching effect on 1-(3,4-dihydroxy benzoyl) pyrene-boric acid complex at 379 nm and 399 nm and the color of its solution was changed from colorless to pale yellow. At 379 nm,the fluorescence intensity change showed a good linear relationship with Cu2+ concentration with linear range of 2.5×10－6-4.0×10－5 mol/L and detection limit of 1.2×10－6 mol/L. The method was applied to the determination of copper in environmental water samples and the recoveries were 97%-99%. The reaction mechanism was tentatively discussed as that the strong binding ability of Cu2+ with 1-(3,4-dihydroxy benzoyl) pyrene caused the decomposition of 1-(3,4-dihydroxy benzoyl) pyrene-boric acid complex and simultaneous formation of 1-(3,4-dihydroxy benzoyl) pyrene-Cu2+ complex,leading to an significant change in the fluorescence and absorption spectrum of its system.
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